Posted by: woodforthetrees | February 25, 2010

Breaking Nature’s NMR barrier

NMR 800 MHz

This is a fantastic time to be an NMR spectroscopist. Image via Wikimedia.

I’m glad to see a letter in today’s Nature commenting on the ‘overly pessimistic’ news feature on 3 February about the first gigahertz nuclear magnetic resonance spectrometer. What should have been a cause for celebration was ruined by a misunderstanding, perhaps even a misrepresentation, of the power and versatility of NMR.

Far from ‘attracting new life to NMR spectroscopy’ — suggesting that the field was on its knees — this investment shows how vibrant this area of research is, at a point when it is poised to make a major contribution to systems biology.

The article describes the world’s most powerful NMR machine, which is now up and running at the European Centre for High Field NMR (CRMN) in Lyon, France. At 1GHz, its magnet is 50 MHz stronger than its nearest rival, and it’s true to say that this a small increase relative to the history of NMR spectrometers, but this misses the point. The question that should have been asked is how powerful are the other machines within that region of France, even within Europe, and what breakthroughs can we expect at this strength?

The Nature news feature mentions that CRMN already has a 900 MHz instrument. But that doesn’t diminish the needed for a stronger magnet. We have barely scratched the surface with regards to structures of membrane proteins, whether they are obtained by crystallography or NMR, and yet these membrane proteins are extremely important for drug research and potential therapy. It’s highly likely that this new machine will begin to make inroads here. But the machine itself can’t solve structures: there are groups around the globe developing techniques that will help produce the structures of larger proteins –look at the paper Science just published.

The worst aspect of this feature is its narrow focus. Yes, NMR works well for small-ish proteins, but the important point is that it offers us opportunities that other techniques don’t. First, it can often produce structures where crystallography has failed. Second, the structure is obtained in solution, allowing us to see a dynamic picture. Third, it’s the only technique that allows us to detect weak, transient interactions, which will be vital for building up a picture of protein-protein and protein-ligand interactions for systems biology and beyond. And fourth, the first in-cell NMR structures were reported about 6 months ago – this will revolutionise our understanding of structures within the cell.

NMR protein spectroscopy is well and truly alive and kicking. So smile, Lyndon Emsley – for goodness sake! You’ve just installed the world’s most powerful NMR machine, worth $16.3 million. In the news feature, you look like you’ve just lost the winning Euro-millions lottery ticket.

Advertisements

Responses

  1. Ha ha!

    Having seen the difference between 600 MHz and 800—and done natural abundance NOESY on both—I am positively *drooling* at the thought of a 1 gig magnet.


Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s

Categories

%d bloggers like this: